2. Histocytochemistry Series Light Microscopic Immunohistochemistry: Fixation and Immunofluorescence Microscopy Using Cryostat-sections (1)
نویسندگان
چکیده
منابع مشابه
Correlative light and immuno-electron microscopy of retinal tissue cryostat sections
Correlative light-electron microscopy (CLEM) is a powerful technique allowing localisation of specific macromolecules within fluorescence microscopy (FM) images to be mapped onto corresponding high-resolution electron microscopy (EM) images. Existing methods are applicable to limited sample types and are technically challenging. Here we describe novel methods to perform CLEM and immuno-electron...
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A new fixation method has been developed for immunofluorescent microscopy using the photosensitive compound 1,3,5-triazido-2,4,6-trinitrobenzene (TTB). Our results show that TTB-fixed cells are well preserved morphologically and that the cellular antigens are better preserved than conventionally fixed cells. By altering one condition at a time in the TTB fixation procedure and analyzing resulti...
متن کاملBHV-1 Antigen Detection in Paraffinized Lung Sections of Pneumonic Sheep Lung Using Immunohistochemistry
Background: Respiratory tract infections caused by some viruses with cattle origin have been demonstrated in sheep and goats. OBJECTIVES: The main goal of this study was to determine Bovine Herpes virus type 1 BHV1antigen in formalin-fixed paraffin-embedded lung tissue of pneumonic sheep, using immunohistochemistry (IHC) staining method. METHODS: For this purpose, the lungs of 4079 sheep, which...
متن کاملImproved double immunohistochemical staining method for cryostat and paraffin wax sections, combining alkaline phosphatase anti-alkaline phosphatase and indirect immunofluorescence.
AIMS To develop and immunohistochemical staining method for cryostat and paraffin wax sections so that two different antigens in the same section of tissues could be detected by combining immunoenzyme and immunofluorescence techniques. METHODS This double immunohistochemical staining method combines alkaline phosphatase-anti-alkaline phosphatase (APAAP) using New Fuchsin as a chromogen and in...
متن کاملStudy of direct immunofluorescence, immunofluorescence mapping and light microscopy in porphyria cutanea tarda.
BACKGROUND Even though porphyria cutanea tarda is the most frequent type of porphyria, there are few studies about its cutaneous physiopathology. OBJECTIVE To evaluate skin changes in porphyria cutanea tarda using light microscopy and direct immunofluorescence before and after treatment with chloroquine. To perform antigen immunomapping of bullae to study their level of cleavage. METHODS Li...
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ژورنال
عنوان ژورنال: Nihon Ika Daigaku Igakkai Zasshi
سال: 2009
ISSN: 1349-8975,1880-2877
DOI: 10.1272/manms.5.136